Single-labeled oligonucleotide probes for homogeneous nucleic acid sequence analysis
US6635427B2 · kind B2 · utility
35Cited by
10References
40Claims
0Family size
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Key dates
| Filing date | Aug 10, 2001 |
| Grant date | Oct 21, 2003 |
| Priority date | — |
| Expiry date | Sep 9, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Probes and methods are provided for detection and analysis of nucleic acid sequences. The probes are single-labeled oligonucleotide probes whose fluorescence emission changes in response to probe-target hybridization and dissociation. The methods are for analyzing one or multiple nucleic acid loci using the probes. This invention further relates to the use of fluorescence changes in single-labeled probes for melting curve analysis, genotyping, and pathogen detection, and to methods for quantification of specific sequences in real-time monitoring of nucleic acid amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.