Multi-sample fermentor and method of using same

US6635441B2 · kind B2 · utility

Assignee

• IRM Inc. · BM

Inventors

• Robert Downs · Ortonville, US
• Scott Lesley · Oregon, US
• James Mainquist · San Diego, US
• Daniel McMullan · San Diego, US
• Andrew Meyer · San Diego, US
• Marc Nasoff · San Diego, US

Key dates

• Filing date: Feb 8, 2001
• Grant date: Oct 21, 2003
• Expiry date: Feb 8, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/818
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A fermentation apparatus is constructed to produce a known and repeatable amount of unpoisoned fermentation product using multiple fermentation vessels. To facilitate further processing compatible with other product processing steps, the fermentation apparatus has an array of sample vessels arranged in a container frame. The container frame is configured to hold the sample vessels during fermentation and to transport the vessel array to or from another processing station. Corresponding to the number of sample vessels in the sample vessel array, a cannula array is configured such that each cannula may be placed inside a sample vessel. The cannula array is attached to a gas distributor that delivers oxygen and/or one or more other gases from a gas source through the cannula into the sample vessel. Because the fermentation volume for each individual sample vessel is smaller than a bulk fermentation apparatus, the fermentation product yields are more predictable and cell growth rates may be more effectively optimized.