Selection for more efficient transformation host cells
US6635457B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Jul 26, 2000 |
| Grant date | Oct 21, 2003 |
| Priority date | — |
| Expiry date | Jul 26, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/19
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods for producing and selecting host cells that better survive transformation treatment by subjecting host cells to conditions that alter them, subjecting the altered cells to transformation conditions, and selecting host cells that survive the transformation conditions. This invention also provides methods for transferring nucleic acids of interest into host cells, using cells that are better able to survive transformation treatment. Also, this invention provides kits for producing or selecting host cells in transformation treatments, as well as, kits comprising various host cells that may be utilized in transformation experiments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.