Fluorescence standard for use in microfluidic instruments
US6635487B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 7, 2001 |
| Grant date | Oct 21, 2003 |
| Priority date | — |
| Expiry date | Oct 30, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/109163
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A test device for use as a fluorescent standard in microfluidic analytical detection systems includes one or more slits that correspond to, and are of similar dimension to, one or more microchannels in a detection region on a corresponding analysis chip. A fluorescent material is attached to the test device on the side opposite the illumination source such that excitation radiation passes through the slit(s), which defines the focal plane of the illumination optics, and impinges on the fluorescent material thereby causing the fluorescent material to fluoresce. By displacing the fluorescent material relative to the focal plane, the intensity of the radiation exciting the fluorescent material is dispersed relative to the intensity of the radiation at the focal plane, and concomitantly the strength of the resulting fluorescent signal is reduced. An optional spacer is provided to increase the distance of the fluorescent material from the focal plane so as to increase the dispersion of the radiation (decrease the intensity impinging on the fluorescent material). The strength of the resulting fluorescent signal from the fluorescent material can be controlled by selecting a spacer with th…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.