Procedure for the simultaneous quantitative and qualitative analysis of both flavonoid glycosides and steroidal glycosides
US6635490B1 · kind B1 · utility
3Cited by
9References
30Claims
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Key dates
| Filing date | May 25, 2000 |
| Grant date | Oct 21, 2003 |
| Priority date | — |
| Expiry date | May 25, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A single rapid procedure for the quantitative and qualitative analysis of flavonoid glycosides and steroidal glycosides, in natural products, has been developed. The material is extracted with aqueous solutions of polar organic solvents in an ultrasonic bath. Interfering compounds are removed by pre-purification procedures prior to instrumental analysis by High Pressure Liquid Chromatography with Negative Ion Electrospray Mass Spectrometric Detection and on-line Diode Array Ultraviolet-Visible Spectroscopy. The analysis of flavonoid and steroidal glycosidic compounds in HOSTA leaves served as a model system.
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