Methods and DNA constructs for gene silencing in transgenic plants
US6635805B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 10, 1999 |
| Grant date | Oct 21, 2003 |
| Priority date | — |
| Expiry date | Nov 10, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/146
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention presents DNA constructs comprising a promoter operably linked to DNA which can be transcribed in a plant cell to an RNA transcript, wherein the RNA transcript comprises plant virus sequence from an RNA virus which confers on the RNA transcript the ability to replicate in the cytoplasm of the plant cell, wherein the transcript lacks all or part of the viral genome not required for replication in the cytoplasm, and further comprises at least one targeting sequence which is foreign to the plant virus sequence and causes post-transcriptional gene silencing of one or more target genes. The invention also presents methods to use the DNA construct to cause post-transcriptional gene silencing of a target gene in a plant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.