Method and apparatus for flow-through hybridization
US6638760B1 · kind B1 · utility
10Cited by
16References
45Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 3, 1998 |
| Grant date | Oct 28, 2003 |
| Priority date | — |
| Expiry date | Dec 3, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.