Analysis method and cuvette therefor

US6638769B2 · kind B2 · utility

Assignee

• Migrata U.K. Limited · CY

Inventors

• Jan E. Lilja · Helsingborg, SE
• Sven-Erik Nilsson · Helsingborg, SE
• Johnny Svensson · Ängelholm, SE
• Annika Eriksson · Helsingborg, SE

Key dates

• Filing date: Oct 10, 2001
• Grant date: Oct 28, 2003
• Expiry date: Oct 10, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/0389
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for hemoglobin determination is described which includes the steps of introducing a sample of undiluted whole blood by capillary action into a disposable microcuvette having at least one cavity for receiving the sample. The cavity includes a dry essentially non-hygroscopic hemolysing agent, which is dissolved by the blood, hemolyses the red blood cells and releases the haemoglobin contained in the blood cells. A first absorption measurement at a wavelength range 490-520 nm is then performed directly on the sample in the microcuvette, and a second absorption measurement is performed to compensate for background interference.