Systems for column-based separations, methods of forming packed columns, and methods of purifying sample components

US6645377B1 · kind B1 · utility

Assignee

• Battelle Memorial Institute · CH

Inventors

• Oleg B. Egorov · West Richland, US
• Matthew J. O'Hara · Richland, US
• Jay W. Grate · West Richland, US
• Darrell P. Chandler · Richland, US
• Fred J. Brockman · Kennewick, US
• Cynthia J. Bruckner-Lea · Richland, US

Key dates

• Filing date: Jul 20, 2000
• Grant date: Nov 11, 2003
• Expiry date: Nov 20, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2030/565
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention encompasses systems for column-based separations, methods of packing and unpacking columns and methods of separating components of samples. In one aspect, the invention includes a system for column-based separations, comprising:a) a fluid passageway, the fluid passageway comprising a column chamber and a flow path in fluid communication with the column chamber, the flow path being obstructed by a retaining material permeable to a carrier fluid and impermeable to a column matrix material suspended in the carrier fluid, the flow path extending through the column chamber and through the retaining material, the flow path being configured to form a packed column within the column chamber when a suspension of the fluid and the column matrix material is flowed along the flow path; andb) the fluid passageway extending through a valve intermediate the column chamber and the retaining material.