Assays for endotoxin
US6645724B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1999 |
| Grant date | Nov 11, 2003 |
| Priority date | — |
| Expiry date | Apr 7, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2799/026
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The horseshoe crab, Carcinoscorpius rotundicauda Factor C cDNA (CrFC21) has been cloned into a shuttle baculoviral vector and another vector suitable for expression in insect cells. The recombinant baculoviral DNA was then transfected into the insect cells for expression of recombinant Factor C. Recombinant Factor C was found to be immunoreactive and is capable of binding both free and bound/immobilized lipid A. It is enzymatically active when triggered by LPS. The rFC is probably of the two-chain form, being cleaved into the heavy and light chains after activation by Gram negative bacterial endotoxin. As low as 0.01 pg (0.001 ng/ml) of LPS was detectable by the rFC, thus, indicating its potentials as a novel generation of “limulus amoebocyte lysate.”
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.