Methods and reagents for the rapid and efficient isolation of circulating cancer cells
US6645731B2 · kind B2 · utility
209Cited by
9References
11Claims
0Family size
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Key dates
| Filing date | Jul 13, 2001 |
| Grant date | Nov 11, 2003 |
| Priority date | — |
| Expiry date | Jul 13, 2021 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01F1/0054
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater sensitivity than conventional PCR or immunohistochemistry by 1-2 orders of magnitude. In addition, the assay facilitates the biological characterization and staging of carcinoma cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.