Containment cuvette for PCR and method of use
US6645758B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 28, 1992 |
| Grant date | Nov 11, 2003 |
| Priority date | — |
| Expiry date | Sep 28, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N35/0098
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A cuvette and a method of use prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released. Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source, or via pistons that are part of the cuvette and operate on the compartments as a piston within a piston chamber.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.