Patent · US Expired

Shuttle vectors

US6645766B2 · kind B2 · utility

2Cited by

4References

4Claims

0Family size

Assignee

Rigel Pharmaceuticals, Inc. · US

Inventors

Ying Luo · Shanghai, CN
Pei Wen Yu · Burlingame, US
James Lorens · Otta, NO

Key dates

Filing date	Jan 7, 2002
Grant date	Nov 11, 2003
Priority date	—
Expiry date	Apr 5, 2022

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/81
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.