Process for delaying the deactivation of glutaryl amidase during enzyme catalysis

US6649369B2 · kind B2 · utility

Assignee

• Aventis Pharma Deutschland GmbH · DE

Inventors

• Jürgen Bongs · Wiesbaden, DE
• Johannes Meiwes · Idstein, DE
• Wolfgang Kruse · Hofheim, DE
• Klaus-Peter Koller · Bad Soden, DE

Key dates

• Filing date: Apr 9, 2002
• Grant date: Nov 18, 2003
• Expiry date: Apr 9, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a process for delaying the deactivation of glutaryl amidase during enzyme catalysis. The enzyme catalyst is, after maximum conversion of the substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the enzyme catalyst loses activity. The inactivation of the enzyme catalyst can be delayed by bringing it into contact with a thiol during the reaction.