Thermophilic alkaline phosphoesterase and its expression
US6649390B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 4, 2000 |
| Grant date | Nov 18, 2003 |
| Priority date | — |
| Expiry date | May 4, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention belongs to the field of biological engineering techniques and relates to a thermophilic alkaline phosphoesterase. The invention provides an amino acid sequence of a thermophilic alkaline phosphoesterase of the invention, as well as a DNA fragment encoding the amino acid sequence. The present invention also includes methods for cloning an expression vector containing the DNA fragment or a portion thereof and for producing the recombinant enzyme. The invention also relates to a method for tagging biological macromolecules utilizing the enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.