Method for determining average solution properties of macromolecules by the injection method
US6651009B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 24, 2002 |
| Grant date | Nov 18, 2003 |
| Priority date | — |
| Expiry date | Jul 24, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/0277
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A new method is presented for measuring the molecular properties of an unfractionated solution of macromolecules. Sharing some similarities with the standard Zimm plot technique, the method begins with the preparation of several sample aliquots spanning a range of concentrations. The aliquots are then injected sequentially into a stream such as provided by a liquid chromatograph. Each aliquot produces, thereby, an effective “peak” whose elements correspond to different concentrations of the diluted aliquot. By analyzing the angular and concentration dependence of the scattering signals throughout the corresponding peaks, the weight averaged molar mass, the mean square radius, and the second virial coefficient may be derived. In contrast to earlier on-line methods, better accuracy is achieved, while using a smaller quantity of sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.