Scaffold formed of tissue treated to eliminate cellular and cytosolic elements
US6652872B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 4, 2001 |
| Grant date | Nov 25, 2003 |
| Priority date | — |
| Expiry date | Apr 4, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/92
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A scaffold for use as a growth supportive base for cells and tissue explants is formed of naturally occurring derived connective or skeletal tissue that has been treated for elimination of cellular and cytosolic components comprising DNA, RNA, peptides, lipids and proteoglycans. The treated tissue can be crosslinked and treated for producing a higher porosity such as by mechanical or chemical treatment. Alternatively, the treated tissue is ground to form flakes having a size below 1000&mgr;, and the flakes are attached to each other to provide a scaffold having a porosity of at least 85%. The scaffold may be composed of several single-flake layers arranged on top of the other. The scaffold can be impregnated with cells to form tissue such as epiphysial tissue, and may contain adhesive molecules or growth factors. A prosthesis for implanting can be formed using the scaffold. The scaffold may be composed of fused epiphyses.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.