High efficiency gene targeting in mouse embryonic stem cells
US6653113B1 · kind B1 · utility
46Cited by
1References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 19, 1999 |
| Grant date | Nov 25, 2003 |
| Priority date | — |
| Expiry date | Feb 19, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/902
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides novel methods for modifying the genome of an animal cell which typically comprise the steps of: constructing a DNA molecule in which desired sequence modifications are contained in a segment of DNA (a “targeting DNA”) that is substantially isogenic with a DNA in the cell genome (a “target DNA”); introducing the targeting DNA construct into the cell (e.g., by microinjection, electroporation, transfection, or calcium phosphate precipitation); and selecting cells in which the desired sequence modifications have been introduced into the genome via homologous recombination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.