Patent · US Expired

Apparatus for, and method of, introducing a substance into an object

US6653136B1 · kind B1 · utility

13Cited by
5References
15Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 25, 2002
Grant dateNov 25, 2003
Priority date—
Expiry dateMar 25, 2022

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12M35/02
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention relates to an apparatus for and method of introducing a substance into an object, particularly into a cell or cellular material. In a preferred arrangement apparatus receives the cell and preferably locates it between first and second electrodes and applies a voltage pulse to cause a disruption in the cell wall. This causes the cell to become permeable. The substance may then be introduced, for example under a fluid pressure. Cells may then be inspected and sorted into transfected and non-transfected types. This may be achieved automatically, for example by using electrophoresis.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.