Method of covalent coupling

US6653457B1 · kind B1 · utility

Assignee

• S2MEDICAL AB · SE

Inventors

• Olle Larm · Bromma, SE
• Ibrahim Gouda · Sollentuna, SE

Key dates

• Filing date: Jun 27, 2002
• Grant date: Nov 25, 2003
• Expiry date: Jun 27, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08B37/0069
• WIPO field: Macromolecular chemistry, polymers
• WIPO sector: Chemistry

Abstract

A method of preparing conjugates by means of covalent coupling of a polysaccharide selected from heparin, heparin derivatives, at least partially deacerylated dermatan sulphate and dextran sulphate and a solid substance containing primary amino groups, said method comprising the following steps: a) reduction of the saccharide so that its terminal monosaccharide unit is converted to an alditol; b) periodate oxidation of the alditol formed in step a) to the formation of a terminal aldehyde group under cleavage of the monosaccharide unit between two vicinal groups selected from hydroxyl and amino groups; and c) reductive coupling of the alditol via the aldehyde group to the amino group of the solid substrate.