Scanning spectrophotometer for high throughput fluroescence detection
US6654119B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 20, 2000 |
| Grant date | Nov 25, 2003 |
| Priority date | — |
| Expiry date | Apr 20, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6482
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.