Hybridization buffers using low molecular weight dextran sulfate and methods for their use
US6656685B2 · kind B2 · utility
42Cited by
5References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 29, 2001 |
| Grant date | Dec 2, 2003 |
| Priority date | — |
| Expiry date | Jan 29, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to an improvement in automated tissue staining system having evaporation inhibitor liquid covering the polynucleotide hybridization buffer-covered tissue on a slide, wherein the improvement comprises a polynucleotide hybridization buffer for in situ hybridization comprising a low molecular weight dextran sulfate having a molecular weight range from about 8,000 to about 16,000.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.