Serum-free, low-protein media for rotavirus vaccine production
US6656719B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 21, 1998 |
| Grant date | Dec 2, 2003 |
| Priority date | — |
| Expiry date | Apr 23, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2720/12351
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Defined serum-free, low protein media (LPKM), that supports 1) Vero cell growth for up to 20 passages, 2) Vero cell growth on microcarriers and 3) rotavirus production is provided. Maximum cell densities attained are 60-100% of that in serum-containing medium; the doubling time is equal to that for cells in serum containing medium. Rotavirus titers achieved in LPKM-1 are 50-100% of the serum-containing process. Finally, since LPKM-1 contains no animal-sourced proteins, the problems associated with the serum-containing rotavirus production process (i.e. lengthy wash steps before infection, potential introduction of adventitious agents and lot-to-lot variability) can be avoided; while maintaining nearly equivalent product titers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.