Method for identification of target genes of transcription factors
US6656735B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 14, 1998 |
| Grant date | Dec 2, 2003 |
| Priority date | — |
| Expiry date | Sep 14, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Transcription factor responsive elements, target genes, and co-factors of a transcription factor, either individually or in combination, are identified by integrating into the genome of a eukaryotic cell a gene trap vector that includes a reporter gene, a polyadenylation site, and a selectable marker gene, selecting the cells in which the gene trap vector is successfully integrated and contacting those cells with the transcription factor, then identifying and cultivating those cells having a gene into which the gene trap vector has been integrated and in which the reporter gene activity has been altered by interaction between the transcription factor and a transcription factor responsive element of that gene, and identifying the transcription factor responsive element, target gene, or cofactor that interacts with the transcription factor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.