Method for composite cell-based implants

US6662805B2 · kind B2 · utility

Assignees

• THE JOHNS HOPKINS UNIVERSITY · US
• Chondros, Inc. · US

Inventors

• Carmelita Frondoza · Elkton, US
• David S. Hungerford · Cockeysville, US
• Alan H. Shikani · Ruxton, US
• Abraham J. Domb · Beer Sheva, IL
• David J. Fink · State College, US
• Leonard Bloom · Owings Mills, US

Key dates

• Filing date: Aug 6, 2001
• Grant date: Dec 16, 2003
• Expiry date: Mar 5, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/72
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention is a method for the implantation of a combination of cells or cell-microcarrier aggregates wherein one component comprises a solid implantable construct and a second component comprises an injectable formulation. For example, in one embodiment, the solid implant may be first implanted to fill the majority of the cavity receiving the implant, and then cells or cell-microcarrier aggregates in an injectable format, with or without the addition of gelling materials to promote rapid gelling in situ, may be injected into spaces surrounding the solid implant in order to secure the solid implant in the site and/or to promote rapid adherence and/or integration of the solid implant to surrounding tissues. Also contemplated in this embodiment is that the cellular composition of the injectable component may differ from that of the solid component. For example, the solid implant may result from the culturing of chondrocytes on microcarriers or scaffolds, thereby resulting in an implant having cartilage-like properties, whereas the injectable cells or aggregates may result from the culturing of stem cells, resulting thereby in cells capable of producing cells of a chondrogenic, fi…