Identification and characterization of interacting molecules

US6664048B1 · kind B1 · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Erich Wanker · Berlin, DE
• David BANCROFT · Gilching, DE
• Hans Lehrach · Berlin, DE
• Niels Wedemeyer · Berlin, DE
• Elmar Maier · Feldkirch, AT
• Sebastian Meier-Ewert · Martinsried, DE

Key dates

• Filing date: May 30, 2000
• Grant date: Dec 16, 2003
• Expiry date: May 30, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/52
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to an improved method for the identification and optionally the characterization of interacting molecules designed to detect positive clones from the rather large numbers of false positive clones isolated by two-hybrid systems. The method of the invention relies on a novel combination of selection steps used to detect clones that express interacting molecules from false positive clones. The present invention further relates to a kit useful for carrying out the method of the invention. The present invention provides for parallel, high-throughput or automated interaction screens for the reliable identification of interacting molecules.