Measuring viral reverse transcriptase activity
US6664065B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | Apr 24, 2002 |
| Grant date | Dec 16, 2003 |
| Priority date | — |
| Expiry date | May 1, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/703
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In clinical settings as well as in a drug-discovery context, the impact of an agent that may affect reverse transcriptase (RT) can be measured and even quantified by bringing a sample, which may contain an RT, into contact with an RNA template, a primer complementary to the RNA template, and appropriate oligonucleotide-specific primers, under conditions such that they react, in the presence of RT, to form a cDNA product in inverse proportion to the effect of the agent. The amount of any resultant cDNA product then can be measured. The approach is readily implemented as a real-time, quantitative kinetic assay for RT activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.