Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides
US6664112B2 · kind B2 · utility
94Cited by
5References
12Claims
0Family size
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Key dates
| Filing date | Jun 1, 2001 |
| Grant date | Dec 16, 2003 |
| Priority date | — |
| Expiry date | Jun 1, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Synthetic oligonucleotides, such as synthetic DNA, often contain sequence errors due to synthetic failures (e.g., side products and/or truncated products). Methods are provided herein for improving the sequence fidelity of synthetic double-stranded oligonucleotides by separative depletion of synthetic failures. Separation is effected by utilization of methodologies in a preparative mode under denaturing conditions. A preferred use of the methods relates to gene synthesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.