Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
US6670128B2 · kind B2 · utility
25Cited by
10References
34Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 15, 2001 |
| Grant date | Dec 30, 2003 |
| Priority date | — |
| Expiry date | Aug 15, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25375
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provide methods for utilizing spent leukodepletion filter devices as a source of material for the isolation and analysis of genomic DNA (gDNA), including polymorphism, genotyping, and pharmacogenomic studies. Cellular retentate with the filter contains leukocytes, which are lysed to release the nuclei. The nuclei are lysed or ruptured to release genomic DNA, which is then isolated and used for subsequent analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.