Patent · US Expired

Cell transfection apparatus and methods for making and using the cell transfection apparatus

US6670129B2 · kind B2 · utility

12Cited by
5References
40Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 24, 2001
Grant dateDec 30, 2003
Priority date
Expiry dateJul 22, 2022

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12M35/02
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A cell transfection apparatus and methods for making and using the cell transfection apparatus are described. The cell transfection apparatus has a surface on which there is printed at different locations one or more foreign biomolecules (e.g., DNA, RNA, oligonucleotides, nucleotides). The foreign biomolecules can be printed at the same time. The surface is then covered with a transfection reagent which is incubated and removed from the surface before cells in a cell growth media are placed on the surface. The surface is configured such that the cells which become transfected with one or more foreign biomolecules are segregated from the cells which fail to become transfected with one or more foreign biomolecules. There are disclosed two embodiments of the cell transfection apparatus.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.