Compositions comprising bioactive peptides prepared without formation of native disulfide bonds
US6670148B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 5, 1999 |
| Grant date | Dec 30, 2003 |
| Priority date | — |
| Expiry date | Aug 5, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/46
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of preparing a bioactive polypeptide in a stable, inactivated form, the method comprising the step of treating the polypeptide with ozonated water in order to oxidize and/or stabilize the cysteine residues, and in turn, prevent the formation of disulfide bridges necessary for bioactivity. The method can involve the use of ozonated water to both oxidize the disulfide bridges in a bioactive polypeptide, and to then stabilize the resultant cysteine residues. Optionally, and preferably, the method can involve the use of ozonated water to stabilize the cysteine residues, and thereby prevent the formation of disulfide bridges, in a polypeptide produced by recombinant means in a manner that allows the polypeptide to be recovered with the disulfide bridges unformed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.