Process for the preparation in pure form of the protease activating blood clotting VII, its proenzyme or a mixture of both proteins by means of affinity chromatography

US6670455B1 · kind B1 · utility

Assignee

• Aventis Behring GmbH · DE

Inventors

• Juergen Roemisch · Marburg, DE
• Annette Feussner · Marburg, DE
• Hans-Arnold Stoehr · Wetter, DE

Key dates

• Filing date: Aug 4, 2000
• Grant date: Dec 30, 2003
• Expiry date: Dec 17, 2020

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01D15/3828
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A process for the preparation in pure form of the protease activating blood clotting factor VII and/or its proenzyme by the use of a chromatography separation processes and/or fractional precipitation is described. The process used may include adsorption on calcium phosphate/hydroxyapatite, a hydrophobic matrix, a chelate matrix, a matrix on which heparin or a substance related to heparin, such as heparin sulfate or dextran sulfate, is immobilized, or a matrix that is coated with an immobilized monoclonal or polyclonal antibody directed against the protein to be isolated, or F(ab) or F(ab)2 fragments of antibodies directed against the protein to be isolated. A pharmaceutical preparation and a reagent are described which contain the said protease and/or its proenzyme.