DNA amplification of a single cell

US6673541B1 · kind B1 · utility

Assignee

• Micromet AG · DE

Inventors

• Christoph Klein · Darmstadt, DE
• Oleg Schmidt-Kittler · Munich, DE

Key dates

• Filing date: Jun 18, 2001
• Grant date: Jan 6, 2004
• Expiry date: Jun 18, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6855
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a novel method for the amplification of DNA, this method being particularly useful for the amplification of the DNA or the whole genome of a single cell, chromosomes or fragments thereof. Described is also the use of the method in DNA analysis for medical, forensic, diagnostic or scientific purposes, like comparative genomic hybridization (CGH)-, fluorescence in situ hybridization (FISH)-, polymerase chain reaction (PCR)-, single strand conformation polymorphism (SSCP)-, DNA sequence-, “loss of heterozygosity” (LOH)-, fingerprint- and/or restriction fragment length polymorphism (RFLP)-analysis.