Method for preparing polypeptides with appropriate glycosilation

US6673575B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Reinhard Franze · Penzberg, DE
• Horst Eberhardt · Eltville am Rhein, DE
• Claus Wallerius · Penzberg, DE

Key dates

• Filing date: Sep 5, 2000
• Grant date: Jan 6, 2004
• Expiry date: Sep 5, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention concerns a process for the production of a polypeptide with suitable glycosylation by culturing eukaryotic cells and isolating the polypeptide from the culture medium or/and the cells. In this process the desired glycosylated polypeptide can be produced recombinantly with the aid of endogenous gene activation or be produced naturally by the cells.