Detection and confirmation of nucleic acid sequences by use of poisoning oligonucleotides
US6673577B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 14, 2000 |
| Grant date | Jan 6, 2004 |
| Priority date | — |
| Expiry date | Nov 14, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a methodology which is directed to providing positive confirmation that nucleic acids, possessing putatively identified sequences predicted to generate observed GeneCalling™ signals, are actually present within the sample from which the signal was originally derived. The putatively identified nucleic acid fragment within the sample possesses 3′- and 5′-ends with known terminal subsequences. The method involves contacting nucleic acid fragments in a sample in amplifying conditions with (i) a nucleic acid polymerase; (ii) “regular” primer oligonucleotides having sequences comprising hybridizable portions of known terminal subsequences; and (iii) a “poisoning” oligonucleotide primer. Nucleic acids amplified with a poisoning primer are distinguishable upon detection from nucleic acids amplified with regular primers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.