&agr;-amylase mutants

US6673589B2 · kind B2 · utility

Assignee

• Novozymes A/S · DK

Inventors

• Torben Vedel Borchert · Nørresundby, DK
• Allan Svendsen · Paseo Los Muchachos, DK
• Carsten Andersen
• Bjarne Ronfeldt Nielsen · Avenue Madeleine, DK
• Torben Lauesgaard Nissen · Summit, US
• Søren Kjærulff · Copenhagen, DK

Key dates

• Filing date: Jan 25, 2001
• Grant date: Jan 6, 2004
• Expiry date: Jan 25, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01001
• WIPO field: Basic materials chemistry
• WIPO sector: Chemistry

Abstract

The invention relates to a variant of a parent Termamyl-like &agr;-amylase, which exhibits an alteration in at least one of the following properties relative to said parent &agr;-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.