Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases

US6677140B2 · kind B2 · utility

Assignee

• Promega Corporation · US

Inventors

• Randall L. Dimond · Madison, US
• Steven J. Ekenberg · Verona, US
• James Robert Hartnett · Fitchburg, US
• Geoffrey R. Hudson · Madison, US
• Leopoldo G. Mendoza · Madison, US
• Katharine M. Miller · Verona, US
• John E. Monahan · Walpole, US
• Christopher Jones · Perth, AU
• Mark Maffitt · Madison, US
• Richard A. Martinelli · Watertown, US
• Edward E. Pahuski · Sun Prairie, US
• James W. Schumm · Madison, US

Key dates

• Filing date: Feb 7, 2002
• Grant date: Jan 13, 2004
• Expiry date: Feb 7, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6853
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention entails methods and kits for carrying them out based on the discovery that an RNA replicase, such as Q&bgr; replicase, has DNA-dependent RNA polymerase (“DDRP”) activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2′-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase.