Process for the preparation in pure form of the protease activating blood clotting factor VII, its proenzyme or a mixture of both proteins by means of ion-exchange chromatography

US6677440B1 · kind B1 · utility

Assignee

• Aventis Behring GmbH · DE

Inventors

• Juergen Roemisch · Marburg, DE
• Annette Feussner · Marburg, DE
• Hans-Arnold Stoehr · Wetter, DE

Key dates

• Filing date: Aug 4, 2000
• Grant date: Jan 13, 2004
• Expiry date: Aug 4, 2020

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A process for the preparation of a pure form of protein, wherein the protein is protease activating blood clotting factor VII, the proenzyme for the protease, or a mixture of both the-protease and the proenzyme, is described. The protein is obtained from biological fluids or through genetic engineering and is isolated by anion- and/or cation-exchange chromatography at a pH below the isoelectric point of the protein to be isolated, or by a combination of anion- or cation-exchange chromatography with a chromatography process and/or fractional precipitation at pH between 2.5 and 9.0.