Method and apparatus for the detection of fluorescent light in confocal scanning microscopy
US6677596B2 · kind B2 · utility
7Cited by
11References
17Claims
0Family size
Assignee
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Key dates
| Filing date | Sep 6, 2001 |
| Grant date | Jan 13, 2004 |
| Priority date | — |
| Expiry date | Feb 13, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/16
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detection of fluorescent light in scanning microscopy includes dividing an illuminating light beam into a plurality of partial illuminating beams so as to illuminate multiple specimen regions simultaneously. Fluorescing materials in a specimen region are excited via multi-photon excitation. Operating parameters of the light source are adapted for optimum fluorescent photon yield to the properties of the fluorescing material in the specimen region. Fluorescent light of the specimen regions is detected simultaneously.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.