In vitro activation of mammalian oocytes
US6680199B1 · kind B1 · utility
7Cited by
3References
22Claims
0Family size
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Key dates
| Filing date | May 22, 2000 |
| Grant date | Jan 20, 2004 |
| Priority date | — |
| Expiry date | May 22, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/999
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process of parthenogenic activation of mammalian oocytes which includes increasing intercellular levels of divalent cations in the oocyte; and reducing phosphorylation of cellular proteins in the oocyte. One method of accomplishing this is by introducing Ca2+free cation, such as ionomycin, to the oocyte and then preventing phosphorylation of the cellular proteins within the oocyte by adding a serine-threonine kinase inhibitor, such as 6-dimethylaminopurine (DMAP).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.