Patent · US Expired

Methods and compositions for controlled polypeptide synthesis

US6680365B1 · kind B1 · utility

32Cited by
0References
25Claims
0Family size

Assignee

Inventor

Key dates

Filing dateMar 19, 1999
Grant dateJan 20, 2004
Priority date
Expiry dateMar 19, 2019

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K1/088
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Methods and compositions for the generation of polypeptides having varied material properties are disclosed herein. Methods include means for making block copolypeptides and related protocols for adding aminoacid-N-carboxyanhydrides (NCAs) to polyaminoacid chains by exposing the NCA to solutions containing polyaminoacid chains having an amido amidate metallacyle end groups and reacting the NCA with the amido containing metallacyle end group so that the NCA is added to the polyaminoacid chain. Addition methods include means of controlling the addition of aminoacid-N-carboxyanhydrides to polyaminoacid chains by reacting NCAs with initiator molecules and allowing initiator complexes to open the ring of the NCAs through oxidative addition across either the O—C5 or O—C2 anhydride bond resulting a controlled polypeptide polymerization. Novel compositions for use in peptide synthesis and design including five and six membered amido-containing metallacycles and block copolypeptides are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.