Substrate and a method for determining and/or monitoring electrophysiological properties of ion channels
US6682649B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 2, 2000 |
| Grant date | Jan 27, 2004 |
| Priority date | — |
| Expiry date | Jul 5, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/48728
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a substrate and a method for obtaining an electrophysiological measuring configuration in which a cell forms a high resistive seal (giga-seal) around a measuring electrode making it suitable for determining and monitoring a current flow through the cell membrane. The substrate is typically part of an apparatus for studying electrical events in cell membranes, such as an apparatus for carrying out patch clamp techniques utilised to study ion transfer channels in biological membranes. The substrate has a plurality or an array of measuring sites with integrated measuring and reference electrodes formed by wafer processing technology. The electrodes are adapted to conduct a current between them by delivery of ions by one electrode and receipt of ions by the other electrode and are typically silver/silver halide electrodes. This allows for effective and fast measuring of cells in configurations where the there is a direct electrical connection between the measuring electrode and the cell interior, a whole-cell measuring configuration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.