Methods and apparatus for the high through-put detection of binding interactions in vivo
US6682927B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 5, 2001 |
| Grant date | Jan 27, 2004 |
| Priority date | — |
| Expiry date | Oct 17, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6456
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for screening for translocation of a first protein of interest in vivo in a plurality of cells comprises (a) a thin unitary total internal reflection member having a surface portion, (b) a plurality cell contacted to said surface portion by the plasma membrane of said cell, said cell containing said first protein of interest, the protein of interest having a fluorescent group conjugated thereto; (c) a light source operatively associated with the total internal reflection member and positioned for directing a source light into the member to produce an evanescent field adjacent the surface portion, with the evanescent field extending into a first portion of the cell adjacent the plasma membrane, the evanescent field being weaker in a second portion of the cell, the fluorescent group emitting light when in the first portion of the cell and emitting less light when in the second portion of the cell; (d) coupling means for coupling the light source to the thin unitary total internal reflection member and illuminate at least 10 square millimeters of the surface portion; and (e) a light detector operatively associated with the total internal reflection member and configured t…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.