Adsorption of polyampholytes to charged surfaces and assays incorporating same

US6686144B2 · kind B2 · utility

Assignee

• The Research Foundation of the State University of New York · US

Inventors

• Kenneth J. McLeod · Setauket, US
• Nadine Pernodet · Huntington Station, US
• Miriam Rafailovich · Plainview, US

Key dates

• Filing date: Mar 21, 2001
• Grant date: Feb 3, 2004
• Expiry date: Jul 12, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Polyampholytes including fibronectin and aggrecan are made to aggregate and form fibrillar lattice networks environment free of cells, extraneous proteins and other materials which may be present in an extracellular matrix. A composition having sufficient charge density is utilized to cause aggregation and self-assembly of such polyampholytes and to provide an assay for determining the effects of environmental agents on such aggregation and self-assembly.