Immunological detection of RNA:DNA hybrids on microarrays
US6686151B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 6, 2000 |
| Grant date | Feb 3, 2004 |
| Priority date | — |
| Expiry date | Jan 29, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.