Screening of phage displayed peptides without clearing of the cell culture
US6686154B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 4, 2001 |
| Grant date | Feb 3, 2004 |
| Priority date | — |
| Expiry date | Jun 4, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1037
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
This invention provides methods for screening populations of phage-displayed polypeptides that are particularly well-suited for high-throughput screening. The methods do not require the clearing of cells from a culture used to obtain the population of phage or other replicable genetic packages. Accordingly, the invention provides methods for forming complexes between a replicable genetic package displaying a polypeptide fusion and a target molecule in an uncleared cell culture containing replicable genetic package. Compositions made up of an uncleared cell culture containing replicable genetic packages displaying a polypeptide fusion and a target molecule are provided in the invention as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.