Methods and compositions for transcription-based nucleic acid amplification
US6686156B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 26, 2001 |
| Grant date | Feb 3, 2004 |
| Priority date | — |
| Expiry date | Jul 2, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification of the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.