Methods and compositions for controlled polypeptide synthesis

US6686446B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Timothy J. Deming · Summerland, US
• Miaoer Yu · San Jose, US
• Scott Curtin · Berkeley, US
• Jungyeon Hwang · Goleta, US
• Michael D. Wyrsta · Lompoc, US
• Andrew P. Nowak · Los Angeles, US
• Scott W. Seidel · Goleta, US

Key dates

• Filing date: Jun 8, 2001
• Grant date: Feb 3, 2004
• Expiry date: Jun 8, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08G69/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions for the generation of polypeptides having varied material properties are disclosed herein. Methods include means for initiating the polymerization of aminoacid-N-carboxyanhydride (NCA) monomer by combining the monomer with an amido-containing metallacycle, for making self assembling amphiphilic block copolypeptides and related protocols for adding oligo(ethyleneglycol) functionalized aminoacid-N-carboxyanhydrides (NCAs) to polyaminoacid chains. Additional methods include means of adding an end group to the carboxy terminus of a polyaminoacid chain by reacting an alloc-protected amino acid amide with a transition metal-donor ligand complex to forming an amido-amidate metallacycle for use in further polymerization reactions. Novel compositions for use in peptide synthesis and design including five and six membered amido-containing metallacycles and block copolypeptides are also disclosed.