Assays for nuclear receptor agonists and antagonists using fluorescence resonance energy transfer
US6689574B1 · kind B1 · utility
9Cited by
6References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 5, 1998 |
| Grant date | Feb 10, 2004 |
| Priority date | — |
| Expiry date | Mar 13, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/70567
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a method of identifying agonists and antagonists of nuclear receptors that comprises measuring agonist-dependent fluorescence resonance energy transfer (FRET) between a fluorescently labeled nuclear receptor or ligand binding domain and fluorescently labeled CREB-binding protein (CBP), p300, other nuclear co-activator, or binding portion thereof. The method is simple, rapid, and inexpensive. Nuclear receptors and nuclear receptor co-activators labeled with fluorescent reagents for use in the above-described method are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.