Thermostable DNA polymerase from anaerocellum thermophilum

US6692932B1 · kind B1 · utility

Inventors

• Waltraud Ankenbauer · Penzberg, DE
• Gudrun Schmitz-Agheguian · Bernried, DE
• Elizaveta Bonch-Osmolovskaya · Moscow, RU
• Vitaly Svetlichny · Bayreuth, DE
• Ursula Markau · Polling, DE
• Bernhard Angerer · Rosenheim, DE
• Astrid Reiser · Antdorf, DE

Key dates

• Filing date: Jun 10, 1999
• Grant date: Feb 17, 2004
• Expiry date: Jun 10, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophiluml. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.